Figure 1
A. The PQ rodents displayed a collapse of pulmonary architecture and edematous interstitial space and severe infiltration of mononuclear cells into intra-alveolar and interstitial spaces. B. The histopathological results demonstrating lung injuries 6hr and 24hr after PQ corresponded to the histopathological specimen (p<0.01 and p<0.05 respectively).
 
Figure 2
The heat map in microarray analysis presented 2287 genes showing statistically considerable differential expressions in PQ rodents at 6h and 24h after PQ when compared to controls.
 
Figure 3
A.       16 profiles containing a cluster of multiple genes with similar expression patterns were identified by STEM software after PQ were identified (Figure 3). Of these clusters, five were comprised of genes that expressed gradually decreasing levels at different points of time, for example profiles 9, 4, 7 13 and 15; while genes in profiles 4 and 1 had reverse effects. B. 6hr and 24 hr after PQ, the profiles of 4 and 7 shared an analogous downward expression.
 
Figure 4
By GO-analysis, in patterns 4 and 7in which genes have descending expression over the course of 6 hr and 24 hr, all the highest enrichments of GO are associated with mitochondron.
 
Figure 5
A. From the total differential genes, 76 (all belonging to STC patterns 4 and 7) were selected as candidate genes as function of mitochondron by constructing a gene co-expression network with k-core algorithm.
B. The PSTK gene is located at the center of 11 k-core subnetwork in the co-expression network of 76 genes of pattern 4 and 7. It directly regulates 30 adjacent genes that interact according to their degrees. Because the clustering coefficients of these genes (which are above 0.99) are higher than other genes, these interactions rely strongly on PSTK.
C and D. A heat map proved that the expression of PSTK mRNA gradually decreased and demonstrated these 31 differential genes.
 
Figure 6
A. There was no statistical significance (p > 0.05) between 24hr and 6hr groups but a real-time RT-PCR proved that the mRNA level of PSTK showed a statistically significant decrease (p < 0.05) in lungs at 6hrs and 24hrs, when contrasted to those from control rodents.
B. Western Blot demonstrated that,as compared with that in normal groups, the PSTK protein level was less expressed in both 6hr and 24hr groups. There was, however, no difference between 6hr and 24hr groups.
 
Figure 7
A. Immunofluorescence staining proved that PSTK in lungs has a lower number in lungs of 6hr and 24hr groups. A. The number of PSTK positive staining (the green dots) from normal control groups is nearly two times more than that from both 6hr and 24hr PQ groups as examined under the HPF magnitude microscopy.
B. Quantification of PSTK immunofluorescence staining in normal control and PQ groups.
 
 
 
 
 
 
 
 
 

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